Gschwendt M, Kittstein W
Mol Cell Endocrinol. 1980 Dec;20(3):251-60. doi: 10.1016/0303-7207(80)90041-6.
Salt treatment of the cytoplasmic estradiol-receptor complex from chick oviduct induces a strong affinity of the complex for DNA-cellulose and phenyl-sepharose. This process is called activation. Binding to heparin- and lysozyme-sepharose is also observed with the untreated complex. But, the salt treatment, additional binding of the complex to these adsorbents is seen. The increased ability of the complex to bind to polyanions and polycations is destroyed by mild trypsination. The binding to the hydrophobic adsorbent is not affected by this treatment. Neither a change of the sedimentation constant nor of the size of the receptor protein is observed after salt treatment in the cold. After binding of the salt-activated estradiol-receptor complex to DNA-cellulose in the cold, an increase of its sedimentation constant and its size, as measured by density-gradient centrifugation and agarose gel chromatography, resp., becomes apparent. A similar phenomenon is observed after binding to DEAE-cellulose and to some extent after binding to heparin-sepharose. The nuclear complex seems to have the same sedimentation constant as the cytoplasmic complex eluted from DNA-cellulose. The sedimentation constant of the nuclear complex is not changed after DNA-cellulose chromatography. The cytoplasmic progesterone-receptor complex from the same tissue, i.e. the oviduct, does not show any change of size. Thus the well-known process of transformation can now be separated into 2 steps. (1) Activation of the estradiol-receptor complex for its binding to various adsorbents in vitro and probably to its acceptor site(s) in vivo. (2) Increase of receptor size. This second step seems to be a special property of the estradiol-receptor complex. Its physiological significance is unclear.
用盐处理来自鸡输卵管的细胞质雌二醇受体复合物，可诱导该复合物对DNA纤维素和苯基琼脂糖产生强烈亲和力。这一过程被称为激活。未处理的复合物也能与肝素琼脂糖和溶菌酶琼脂糖结合。但是，经过盐处理后，该复合物与这些吸附剂的结合会增加。复合物与聚阴离子和聚阳离子结合能力的增强会被轻度胰蛋白酶处理破坏。而这种处理不会影响其与疏水吸附剂的结合。低温下进行盐处理后，未观察到受体蛋白的沉降常数或大小发生变化。在低温下，盐激活的雌二醇受体复合物与DNA纤维素结合后，通过密度梯度离心和琼脂糖凝胶色谱法分别测量，其沉降常数和大小均明显增加。与DEAE纤维素结合后也观察到类似现象，与肝素琼脂糖结合后在一定程度上也有此现象。核复合物的沉降常数似乎与从DNA纤维素上洗脱下来的细胞质复合物相同。经过DNA纤维素色谱法后，核复合物的沉降常数没有改变。来自同一组织即输卵管的细胞质孕酮受体复合物的大小没有任何变化。因此，现在可以将这个众所周知的转化过程分为两个步骤。（1）激活雌二醇受体复合物，使其在体外与各种吸附剂结合，并可能在体内与受体位点结合。（2）受体大小增加。第二步似乎是雌二醇受体复合物的一个特殊性质。其生理意义尚不清楚。
