鸡输卵管细胞质雌激素受体的DNA结合能力及部分纯化

DNA-binding ability and partial purification of the cytoplasmic estrogen receptor from chicken oviduct.

Gschwendt M

Biochim Biophys Acta. 1980 Feb 7;627(3):281-9. doi: 10.1016/0304-4165(80)90459-6.

Abstract

Large chicken oviducts containing an optimal amount of the cytoplasmic estrogen receptor can be obtained by the application and short-term withdrawal of diethylstilbestrol implants to chickens. 2. The cytoplasmic estrogen-receptor complex from chicken oviduct is able to bind to DNA. The efficiency of the binding (maximal 60%) is dependent on the temperature and the presence of the hormone. 3. The estrogen-receptor complex eluted from the DNA-cellulose by high salt is different from that of untreated cytosol. The observed increase in sedimentation coefficient, molecular weight and asymmetry of the receptor molecule resembles the 'transformation' of the estrogen-receptor complex from rat uterus. 4. Using DNA-cellulose and agarose gel chromatography, the cytoplasmic estrogen-receptor complex from chicken oviduct can be purified 4000-fold.

摘要

通过对鸡植入己烯雌酚并短期撤药，可获得含有适量细胞质雌激素受体的大型鸡输卵管。2. 鸡输卵管的细胞质雌激素受体复合物能够与DNA结合。结合效率（最高60%）取决于温度和激素的存在。3. 用高盐从DNA纤维素上洗脱下来的雌激素受体复合物与未处理的胞质溶胶不同。观察到的受体分子沉降系数、分子量和不对称性的增加类似于大鼠子宫中雌激素受体复合物的“转化”。4. 使用DNA纤维素和琼脂糖凝胶色谱法，鸡输卵管的细胞质雌激素受体复合物可被纯化4000倍。