Stevens J, Stevens Y W, Sloan E, Rosenthal R, Rhodes J
Endocr Res Commun. 1978;5(2):91-108. doi: 10.3109/07435807809089011.
A reliable procedure is described for isolating 3H-triamcinolone acetonide (3H-TA) receptor complexes from purified chronic lymphatic leukemia (CLL) lymphocyte nuclei, based on the use of carbobenzoxy-L-phenylalanine (CBZ-L-phe) to prevent breakdown of hormone-receptor complexes during extraction of nuclei with 0.6M KCl. Using this method, specific nuclear glucocorticoid binding was demonstrated in 14/14 patients with untreated CLL. No correlation was found between levels of nuclear-associated 3H-TA and peripheral white blood cell count or rosetting ability of circulating lymphocytes.
本文描述了一种可靠的方法，可从纯化的慢性淋巴细胞白血病（CLL）淋巴细胞核中分离出3H-曲安奈德（3H-TA）受体复合物。该方法基于使用苄氧羰基-L-苯丙氨酸（CBZ-L-phe）来防止在用0.6M KCl提取细胞核过程中激素-受体复合物的分解。使用该方法，在14例未经治疗的CLL患者中均证实了特异性核糖皮质激素结合。未发现核相关3H-TA水平与外周白细胞计数或循环淋巴细胞的玫瑰花结形成能力之间存在相关性。
