Drapier J C, Quetier F, Petit J F
Eur J Biochem. 1978 Nov 2;91(1):163-70. doi: 10.1111/j.1432-1033.1978.tb20948.x.
Growth conditions and a purification procedure for mycobacteriophage ATCC 11759, a lytic phage for Mycobacterium smegmatis, are described. The phage is a large DNA phage with a very long tail (240 nm). A study of its DNA revealed three interesting features. 1. After denaturation the DNA molecule yields two strands of different buoyant densities. 2. The native DNA has unusual physical properties: its buoyant density in CsC1 is very low (1.654 g/cm3), its sedimentation rate is lower than expected for the molecular weight, its thermal stability at low ionic strength is high. 3. The DNA (in its native form or after reannealing) is resistant to various restriction endonucleases.
描述了耻垢分枝杆菌裂解性噬菌体ATCC 11759的生长条件和纯化程序。该噬菌体是一种大型DNA噬菌体，具有很长的尾部（240纳米）。对其DNA的研究揭示了三个有趣的特征。1. 变性后，DNA分子产生两条具有不同浮力密度的链。2. 天然DNA具有不寻常的物理性质：其在CsC1中的浮力密度非常低（1.654克/立方厘米），沉降速率低于根据分子量预期的值，在低离子强度下热稳定性高。3. DNA（天然形式或复性后）对各种限制性内切酶具有抗性。
