Simeonova M, Tchacarov E
Hum Genet. 1980;56(1):63-6. doi: 10.1007/BF00281570.
Attempts to achieve chromosomal banding by removal of histone proteins by acid or fixation procedures have failed up to now. Numerous biochemical investigations have shown that heparin has a strong and specific affinity to histone proteins. We describe a procedure by which treatment with heparin of nonfixed and fixed metaphase chromosomes leads to G banding; in the former case the effect is observed after a few minutes of heparin treatment; in the latter, much higher concentrations of heparin and 18--24 h incubation are needed. The morphologic effects of heparin treatment, the gradual disruption of chromosomal and nuclear chromatin, are associated with progressive reduction of histone detection (until completely negative) by the alkaline fast green test.
迄今为止，通过酸或固定程序去除组蛋白来实现染色体显带的尝试均告失败。大量生化研究表明，肝素对组蛋白具有强烈且特异的亲和力。我们描述了一种方法，即对未固定和固定的中期染色体用肝素处理可导致G显带；在前一种情况下，肝素处理几分钟后即可观察到效果；在后一种情况下，则需要更高浓度的肝素并孵育18 - 24小时。肝素处理的形态学效应，即染色体和核染色质的逐渐破坏，与碱性固绿试验中组蛋白检测的逐渐减少（直至完全阴性）相关。
