Kangasaho M, Metsä-Ketelä T, Vapaatalo H
Eur J Pharmacol. 1978 Nov 1;52(1):93-8. doi: 10.1016/0014-2999(78)90025-0.
A significant stimulatory action of PGE1 on adenylate cyclase of the particulate membrane preparation of rat heart was observed at 1 X 10(-4) M. PGE1 caused a positive inotropic effect on isolated spontaneously beating rat atria 1 X (10(-5) M, and an increase in atrial cyclic AMP level at 1 X 10(-6) M. PGE2 stimulated the atrial but not the whole heart particulate adenylate cyclase. PGF 2alpha had no effect on these enzyme preparations. Our results suggest that: (i) The inotropic response of isolated spontaneously beating rat atria to PGE1 may be connected with an increase in cyclic AMP level; (ii) the effect of prostaglandins on heart in vivo must be indirect, because the concentrations needed to stimulate isolated atria and particulate adenylate cyclase were high, compared with the doses effective in vivo.
在1×10⁻⁴M时观察到PGE1对大鼠心脏微粒体膜制剂的腺苷酸环化酶有显著的刺激作用。PGE1在1×10⁻⁵M时对离体自发搏动的大鼠心房产生正性肌力作用，在1×10⁻⁶M时使心房环磷酸腺苷水平升高。PGE2刺激心房微粒体腺苷酸环化酶，但不刺激全心微粒体腺苷酸环化酶。PGF2α对这些酶制剂无作用。我们的结果表明：（i）离体自发搏动的大鼠心房对PGE1的正性肌力反应可能与环磷酸腺苷水平升高有关；（ii）前列腺素对体内心脏的作用一定是间接的，因为与体内有效剂量相比，刺激离体心房和微粒体腺苷酸环化酶所需的浓度很高。
