High pressure liquid chromatographic determination of sodium fluoroacetate (compound 1080) in canine gastric content.

A sensitive high pressure liquid chromatographic method was developed for the determination of sodium fluoroacetate (compound 1080) in canine gastric content. The procedure involves extraction of 1080 with water, methyl ethyl ketone, and dilute base, followed by sample cleanup using octadecylsilane bonded phase cartridges and derivatization in ethyl acetate sodium with O-p-nitrobenzyl-N-N'-diisopropylisourea (PNBDI). The compound was chromatographed on a 10 micrometer silica column, and ultraviolet absorbance at 254 and 280 nm was measured. Recovery was greater than 95% for standard 1080 and in the 70-90% range for spiked samples (1-50 ppm).