Mayberry W R
J Bacteriol. 1980 Aug;143(2):582-7. doi: 10.1128/jb.143.2.582-587.1980.
Acid hydrolysates of 140 strains, representing 11 species of the genus Bacteroides, were analyzed by capillary gas-liquid chromatography for total cellular fatty acid. All samples contained components which appeared to be hydroxy fatty acid. The relative amount and chain length distribution of the hydroxy fatty acids, as well as the nonhydroxy fatty acids, varied according to species. To characterize the presumed hydroxy acids, a composite of some 40 of these samples was analyzed by thin-layer and capillary gas-liquid chromatography, mass spectrometry, infrared spectrophotometry, and polarimetry. The hydroxy acids were shown to be of the D-(--)-3-hydroxy acid family. The predominant component was the iso-branched D-(--)-3-hydroxy-15-methylhexadecanoic acid. Lesser amounts of the iso-branched 15-carbon, straight-chain 16-carbon, and anteiso-branched 17-carbon acids were also found.
对代表拟杆菌属11个种的140个菌株的酸性水解产物进行了毛细管气液色谱分析，以测定其总细胞脂肪酸含量。所有样品均含有似乎是羟基脂肪酸的成分。羟基脂肪酸以及非羟基脂肪酸的相对含量和链长分布因菌种而异。为了鉴定推测的羟基酸，对约40个这样的样品的混合物进行了薄层色谱、毛细管气液色谱、质谱分析、红外分光光度法和旋光测定。结果表明，这些羟基酸属于D-(-)-3-羟基酸家族。主要成分是异支链D-(-)-3-羟基-15-甲基十六烷酸。还发现了少量的异支链15碳、直链16碳和前异支链17碳的酸。
