Durham D R, Ornston L N
J Bacteriol. 1980 Aug;143(2):834-40. doi: 10.1128/jb.143.2.834-840.1980.
Intergeneric comparison of the three enzymes that initiate metabolism of protocatechuate in Azotobacter and Pseudomonas species revealed close immunological relatedness of isofunctional proteins. Furthermore, beta-ketoadipate induces all of the enzymes of the protocatechuate pathway (except protocatechuate oxygenase) in Azotobacter and in Pseudomonas species of the "fluorescent" and "cepacia" groups. This regulatory property sets the organisms apart from other bacteria. Protocatechuate oxygenase from Pseudomonas cepacia, like the enzyme from fluorescent Pseudomonas species, cross-reacts strongly with antiserum prepared against protocatechuate oxygenase from Azotobacter vinelandii. Double-diffusion experiments conducted with the antiserum revealed relatedness of Azotobacter spp. Protocatechuate oxygenases in the following order: A. vinelandii = Azotobacter miscellum greater than Azotobacter chroococcum greater than Azotobacter beijerinkii. The antiserum also revealed serological heterogeneity among Pseudomonas spp. protocatechuate oxygenases which were serologically indistinguishable in earlier studies using Pseudomonas aeruginosa protocatechuate oxygenase as reference protein.
对固氮菌属和假单胞菌属中引发原儿茶酸代谢的三种酶进行属间比较，结果显示同功能蛋白具有密切的免疫相关性。此外，β-酮己二酸可诱导固氮菌属以及“荧光”和“洋葱伯克霍尔德菌”组的假单胞菌属中的原儿茶酸途径的所有酶（原儿茶酸加氧酶除外）。这种调节特性使这些生物体与其他细菌区分开来。洋葱伯克霍尔德菌的原儿茶酸加氧酶与荧光假单胞菌属的酶一样，与针对维氏固氮菌的原儿茶酸加氧酶制备的抗血清发生强烈交叉反应。用该抗血清进行的双向扩散实验揭示了固氮菌属原儿茶酸加氧酶的相关性，顺序如下：维氏固氮菌 = 杂色固氮菌 ＞ 球形固氮菌 ＞ 拜氏固氮菌。该抗血清还揭示了假单胞菌属原儿茶酸加氧酶之间的血清学异质性，而在早期以铜绿假单胞菌原儿茶酸加氧酶作为参考蛋白的研究中，这些酶在血清学上是无法区分的。
