De Vries E, Van der Weij J P, Van Buysen A C, Cats A
J Immunol Methods. 1980;36(1):81-7. doi: 10.1016/0022-1759(80)90097-6.
A method is described which permits continuous light microscopic observation of cell cultures under sterile conditions. Moreover, cells or groups of cells selected in such cultures may be processed for electron microscopy, without disturbance of possible cellular interactions, in such a way that these selected cells may be observed in the electron microscope. The method is based on the use of a transparent Teflon film in culture chambers.
本文描述了一种方法，该方法能够在无菌条件下对细胞培养物进行连续的光学显微镜观察。此外，在此类培养物中挑选出的细胞或细胞群，可在不干扰可能存在的细胞间相互作用的情况下，进行电子显微镜样本制备，从而能够在电子显微镜下观察这些挑选出的细胞。该方法基于在培养室中使用透明的聚四氟乙烯薄膜。
