哺乳动物成肌细胞系中M-肌酸激酶存在的证明。
Demonstration of the presence of M-creatine kinase in mammalian myogenic cell lines.
作者信息
Perriard J C, Eppenberger H M
出版信息
Experientia. 1978 Sep 15;34(9):1149-51. doi: 10.1007/BF01922924.
PMID:720508
Abstract
Unequivocal identification of M-CK in cell extracts from fused cells of myogenic cell lines is difficult due to almost identical behaviour of the M-CK and a contaminating enzyme activity in electrophoresis. If CK dimers present in cell extracts were subjected to dissociation and reassociation in the presence of exogenous B-CK subunits, the formation of easily identifiable MB-CK was demonstrated, indicating the presence of M-CK in the myogenic rat cell lines.
摘要
由于在电泳中M-CK和一种污染性酶活性的行为几乎相同，因此很难明确鉴定来自成肌细胞系融合细胞的细胞提取物中的M-CK。如果使细胞提取物中存在的CK二聚体在外源B-CK亚基存在的情况下进行解离和重新缔合，就会证明形成了易于鉴定的MB-CK，这表明在大鼠成肌细胞系中存在M-CK。
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