Kattermann R, Krieger R
J Clin Chem Clin Biochem. 1981 Jan;19(1):31-4.
a micromethod is described for the determination of free or glycoprotein-bound N-acetylneuraminic acid (NANA) on the basis of oxidation with periodic acid and reaction with thiobarbiturate. When using athe Eppendorf microliter system the method is easy to perform, the lowest level of sensitivity being 0.2 nmoles NANA in the incubation mixture. The intraassay precision comprises 3.0% CV, the interassay precision 4.3% CV. The accuracy of the results obtained by this micromethod was proved by comparative experiments with the macromethod according to Warren ((1959), J. Biol. Chem. 234, 1971-1979) using sialyllactose, transferrin and fibrinogen as reference materials.
描述了一种微量方法，用于基于高碘酸氧化和与硫代巴比妥酸盐反应来测定游离的或糖蛋白结合的N-乙酰神经氨酸（NANA）。当使用艾本德微升系统时，该方法易于操作，孵育混合物中NANA的最低检测限为0.2纳摩尔。批内精密度的变异系数（CV）为3.0%，批间精密度的CV为4.3%。通过使用唾液酸乳糖、转铁蛋白和纤维蛋白原作为参考材料，与根据沃伦（（1959年），《生物化学杂志》234卷，1971 - 1979页）的宏观方法进行对比实验，证明了该微量方法所得结果的准确性。
