两种单胺氧化酶对β-苯乙胺的氧化作用：对八个物种脑和肝线粒体中酶的检测

Oxidation of beta-phenylethylamine by both types of monoamine oxidase: examination of enzymes in brain and liver mitochondria of eight species.

作者信息

Suzuki O, Katsumata Y, Oya M

出版信息

J Neurochem. 1981 Mar;36(3):1298-301. doi: 10.1111/j.1471-4159.1981.tb01734.x.

DOI:10.1111/j.1471-4159.1981.tb01734.x

PMID:7205271

Abstract

beta-Phenylethylamine (PEA) was characterized as a substrate for type A and type B monoamine oxidase (MAO) in brain and liver mitochondria of eight species at different substrate concentrations. In all species, at 10.0 microM, PEA was almost specific for type B MAO. At 1000 microM, however, the amine was common for both types of MAO in rat brain and liver, human brain and liver, mouse brain, guinea pig brain and liver, and bovine brain, while it was specific for type B MAO in mouse liver, rabbit brain and liver, bovine liver, pig brain and liver, and chicken brain and liver. From the present study, when PEA is used as a type B substrate, it is recommended that the substrate concentration should be sufficiently low to avoid the effects of species and tissue differences.

摘要

在不同底物浓度下，对8个物种脑和肝线粒体中的β-苯乙胺（PEA）作为A型和B型单胺氧化酶（MAO）的底物进行了特性分析。在所有物种中，10.0微摩尔时，PEA几乎对B型MAO具有特异性。然而，在1000微摩尔时，该胺在大鼠脑和肝、人脑和肝、小鼠脑、豚鼠脑和肝以及牛脑中对两种类型的MAO都适用，而在小鼠肝、兔脑和肝、牛肝、猪脑和肝以及鸡脑和肝中，它对B型MAO具有特异性。从本研究来看，当将PEA用作B型底物时，建议底物浓度应足够低，以避免物种和组织差异的影响。