Levin J A, Wilson S E
J Pharmacol Exp Ther. 1981 Apr;217(1):162-9.
Pretreatment of rabbits with 6-hydroxydopamine (6-OHDA; 25 mg/kg i.v. on day 0 plus 50 mg/kg i.v. on day 1, sacrifice on day 4) depleted 95% of the endogenous catecholamines from the rabbit thoracic aorta and heart. A partial recovery of endogenous catecholamines occurred over the next 2 weeks. 6-OHDA pretreatment decreased the cocaine-sensitive accumulation and the binding of l-[3H]norepinephrine (l-[3H]NE) by 85% in the rabbit thoracic aorta. Thus, this pretreatment with 6-OHDA produced an adequate chemical sympathectomy of the rabbit thoracic aorta. The effects of 6-OHDA pretreatment on the accumulation ane metabolism of l-[3H]NE were examined in the isolated adventitia and isolated media of the rabbit aorta to determine the origin of the O-methylated metabolites formed by the isolated adventitia. In the isolated adventitia, 6-OHDA pretreatment decreased the accumulation of l-[3H]NE and the formation of deaminated metabolites by 80%, but the formation of [3H]normetanephrine was increased more than 2-fold. 6-OHDA produced only a slight decrease in the formation of one of the deaminated metabolites of l-[3H]NE by the isolated media. These results are interpreted to mean that in the isolated adventitia most of the accumulation and deamination of l-[3H]NE occurs in the adrenergic nerve terminals whereas most of the O-methylation occurs in the extraneuronal elements of the isolated adventitia.
用6-羟基多巴胺（6-OHDA；第0天静脉注射25mg/kg，第1天静脉注射50mg/kg，第4天处死）对家兔进行预处理，可使家兔胸主动脉和心脏内95%的内源性儿茶酚胺耗竭。在接下来的2周内，内源性儿茶酚胺出现部分恢复。6-OHDA预处理使家兔胸主动脉中可卡因敏感性积聚和l-[3H]去甲肾上腺素（l-[3H]NE）的结合减少了85%。因此，这种6-OHDA预处理对家兔胸主动脉产生了充分的化学性交感神经切除术。在兔主动脉的分离外膜和分离中膜中研究了6-OHDA预处理对l-[3H]NE积聚和代谢的影响，以确定分离外膜形成的O-甲基化代谢产物的来源。在分离外膜中，6-OHDA预处理使l-[3H]NE的积聚和脱氨代谢产物的形成减少了80%，但[3H]去甲变肾上腺素的形成增加了2倍多。6-OHDA对分离中膜产生的l-[3H]NE的一种脱氨代谢产物的形成仅产生轻微降低。这些结果被解释为意味着在分离外膜中，l-[3H]NE的大部分积聚和脱氨发生在肾上腺素能神经末梢，而大部分O-甲基化发生在分离外膜的神经外成分中。
