Sugiura M, Ohno H, Kunihisa M, Hirata F, Ito H
Jpn J Pharmacol. 1980 Aug;30(4):503-13. doi: 10.1254/jjp.30.503.
A water-soluble polysaccharide, D-II with marked antitumor activity was isolated from the cultured mycelium of Coriolus versicolor by extraction with hot-water, fractional precipitation with ethanol and ion-exchange chromatography. D-II strongly inhibited the growth of Sarcoma-180 transplanted subcutaneously in mice by intraperitoneal, intravenous, subcutaneous or intra-muscular administration at a dose of 5 mg/kg. the molecular weight was estimated to be 2,000,000 by gel-filtration or 6,500,000 by light scattering analysis. The chemical structure of D-II was then investigated by periodate oxidation, methylation analysis, Smith degradation, and a combination of controlled Smith degradation and methylation analysis. These studies proposed that D-II is composed of a unit structure of four D-glucose residues, and is a glucan consisting of beta-D-1,3-linked main chain in which one for every three D-glucose residues is branched at C-6 with beta-D-1,6-linkage.
从云芝培养菌丝体中通过热水提取、乙醇分级沉淀和离子交换色谱法分离出一种具有显著抗肿瘤活性的水溶性多糖D-II。D-II以5mg/kg的剂量通过腹腔、静脉、皮下或肌肉注射，强烈抑制小鼠皮下移植的肉瘤180的生长。通过凝胶过滤法估计分子量为2,000,000，通过光散射分析估计为6,500,000。然后通过高碘酸氧化、甲基化分析、史密斯降解以及受控史密斯降解和甲基化分析相结合的方法研究D-II的化学结构。这些研究表明，D-II由四个D-葡萄糖残基的单元结构组成，是一种葡聚糖，由β-D-1,3连接的主链组成，其中每三个D-葡萄糖残基中有一个在C-6处通过β-D-1,6连接分支。
