一种用于检测苯环利定及其代谢物的气-液色谱分析法。

A gas-liquid chromatography assay for phencyclidine and its metabolites.

作者信息

Kammerer R C, Di Stefano E, Schmitz D

出版信息

J Anal Toxicol. 1980 Nov-Dec;4(6):293-8. doi: 10.1093/jat/4.6.293.

DOI:10.1093/jat/4.6.293

PMID:7206659

Abstract

A GLC assay for phencyclidine (PCP) is described, which also simultaneously measures three primary hydroxylated metabolites formed from incubating PCP in tissue homogenates. Using the FID detector, the limits of reliable detection of PCP and both monohydroxy metabolites, 4-phenyl-4-piperidino-cyclohexanol, 2, and 1-(1-phenylcyclohexyl)-4-hydroxypiperidine, 3, are 0.02 mumol per injection and 0.05 mumol for the dihydroxy metabolite, 4-(4'-hydroxypiperidino)-4-phenylcyclohexanol, 2A. Baseline separation of an compounds was achieved and coefficients of variation (between-run) was 3-6% for PCP, and both monohydroxy metabolites, and 12% for the dihydroxy metabolite. A GCMS assay is also reported herein for the analysis of PCP at low levels, and can detect 5 pmol per injection of PCP, with a linear standard curve from 50 to 2000 pmol.

摘要

本文描述了一种用于检测苯环己哌啶（PCP）的气相色谱法，该方法还能同时测定PCP在组织匀浆中孵育形成的三种主要羟基化代谢产物。使用氢火焰离子化检测器（FID），每次进样时PCP及其两种单羟基代谢产物（4-苯基-4-哌啶基环己醇，2，和1-（1-苯基环己基）-4-羟基哌啶，3）的可靠检测限为0.02 μmol，二羟基代谢产物4-（4'-羟基哌啶基）-4-苯基环己醇，2A的可靠检测限为0.05 μmol。实现了所有化合物的基线分离，PCP及其两种单羟基代谢产物的变异系数（批间）为3-6%，二羟基代谢产物的变异系数为12%。本文还报道了一种用于低水平PCP分析的气相色谱-质谱联用（GCMS）方法，该方法每次进样可检测5 pmol的PCP，标准曲线在50至2000 pmol范围内呈线性。