Bourgeois S
J Supramol Struct. 1980;13(3):401-10. doi: 10.1002/jss.400130311.
The glucocorticoid-induced lysis of lymphoid cell lines offers a genetic approach to steroid hormone action because unresponsive variants can easily be selected as resistant to this lytic effect. The present state of analysis of lymphocytolysis in two murine cell lines, the S49 T-lymphoma and the W7 thymoma, is reviewed. All glucocorticoid-resistant variants isolated so far result from various defects in the glucocorticoid receptor. The absence of variants blocked at another step of the lytic mechanism is discussed. The observed hemizygosity of the glucocorticoid receptor locus in the S49 line and the instability of cell hybrids illustrate some of the potential problems encountered in somatic cell genetics.
糖皮质激素诱导的淋巴样细胞系裂解为研究类固醇激素作用提供了一种遗传学方法，因为可以很容易地筛选出对这种裂解效应具有抗性的无反应变异体。本文综述了在两种小鼠细胞系，即S49 T淋巴瘤细胞系和W7胸腺瘤细胞系中淋巴细胞溶解分析的现状。迄今分离出的所有糖皮质激素抗性变异体均源于糖皮质激素受体的各种缺陷。文中讨论了在裂解机制的其他步骤受阻的变异体缺失的情况。在S49细胞系中观察到的糖皮质激素受体基因座的半合子状态以及细胞杂种的不稳定性，说明了体细胞遗传学中遇到的一些潜在问题。
