Wyse D G
J Pharmacol Methods. 1980 Dec;4(4):313-26. doi: 10.1016/0160-5402(80)90051-0.
Responses of helical strips of ventral tail (muscular) and anterior mesenteric (elastic) artery of rats of two different sizes to a fixed concentration of norepinephrine (NE, 6 X 10(-7)M) were plotted versus incremental levels of resting tension. In addition, complete NE cumulative dose-response curves (6 X 10(-10) to 6 X 10(-4)M) were obtained at the optimal resting tension of each strip. Thirteen measurements or calculations of strip dimensions were made on each vessel and active developed force was divided by each of the dimensions. Vessels from larger rats had thicker walls. The active versus resting tension curve for the muscular artery was steeper and thus determination of optimal resting tension is more critical in this artery. Among the measurements used, active developed force of the muscular artery strip was best "normalized" for variation in wall thickness when expressed as percentage or force per unit of total or tunica media cross-sectional area. Active developed force of the elastic artery strip was best "normalized" for variation in wall thickness when expressed as percentage or per unit of total volume. NE dose-response curves have substantially different appearances when responses are expressed in alternative ways. In conclusion, careful attention to these technical details is necessary in comparison of active developed force in arteries of different sizes. Different techniques may be needed for muscular and elastic arteries.
将两种不同大小大鼠的腹侧尾（肌肉性）动脉和肠系膜前（弹性）动脉的螺旋条带对固定浓度去甲肾上腺素（NE，6×10⁻⁷M）的反应与静息张力的增量水平作图。此外，在每条条带的最佳静息张力下获得完整的NE累积剂量-反应曲线（6×10⁻¹⁰至6×10⁻⁴M）。对每条血管进行13次条带尺寸的测量或计算，并将主动产生的力除以每个尺寸。来自较大大鼠的血管壁更厚。肌肉性动脉的主动与静息张力曲线更陡峭，因此在该动脉中确定最佳静息张力更为关键。在所使用的测量中，当以百分比或每单位总或中膜横截面积的力表示时，肌肉性动脉条带的主动产生的力对于壁厚变化的“标准化”效果最佳。当以百分比或每单位总体积表示时，弹性动脉条带的主动产生的力对于壁厚变化的“标准化”效果最佳。当以不同方式表示反应时，NE剂量-反应曲线具有显著不同的外观。总之，在比较不同大小动脉的主动产生的力时，必须仔细关注这些技术细节。肌肉性动脉和弹性动脉可能需要不同的技术。
