Blackett A D, Hall D A
Mech Ageing Dev. 1980 Nov-Dec;14(3-4):305-16. doi: 10.1016/0047-6374(80)90004-4.
In an attempt to determine the relevance of the free radical theory of ageing to age changes discernible in connective tissue parameters, a small colony of C3H/He and LAF1 mice was set up, with sample culled at intervals throughout the lifespan to provide experimental samples. To half of the stock a dietary supplement of vitamin E, a naturally occurring antioxidant, was given at a level of 2500 mg/kg of diet. Tests were carried out on culled samples to provide data on the total collagen levels of bone and skin, on thermal shrinkage temperature and maximal degree of shrinkage of tendon fibres, and on the recovery of skin from stress. Vitamin E was found to have no effect on any of the parameters measured on C3H/He mice but to exert an influence on the parameters of LAF1 mice around the age of 10 months. This influence, however, is not regarded as being relevant to the ageing of the tissues and thus no evidence can be derived for a free-radical mechanism playing a role in the ageing of connective tissues.
为了确定衰老的自由基理论与结缔组织参数中可辨别的年龄变化之间的相关性，建立了一小群C3H/He和LAF1小鼠，并在其整个寿命期间定期采集样本以提供实验样本。给一半的小鼠喂食含2500 mg/kg饮食水平的天然抗氧化剂维生素E的膳食补充剂。对采集的样本进行测试，以提供有关骨骼和皮肤中总胶原蛋白水平、肌腱纤维的热收缩温度和最大收缩程度以及皮肤从应激中恢复的数据。发现维生素E对C3H/He小鼠测量的任何参数均无影响，但对10个月龄左右的LAF1小鼠的参数有影响。然而，这种影响被认为与组织衰老无关，因此无法得出自由基机制在结缔组织衰老中起作用的证据。
