Seale T W, Flux M, Rennert O M, Quissell D O, Barzen K A, Lafferty J L
Pediatr Res. 1980 Dec;14(12):1398-402. doi: 10.1203/00006450-198012000-00029.
The action of serum from cystic fibrosis patients on the intracellular maintenance, efflux, and uptake of potassium ions in dispersed acinar cells from the rat submandibular gland, a seromucous salivary gland, was investigated in an attempt to demonstrate the presence of factors affecting ion-coupled secretion. No effects specific to cystic fibrosis serum were demonstrated upon the following parameters of potassium transport: (1) maximal stimulation of K+ efflux by the cholinergic agonists carbamylcholine; (2) the dose-response curve of K+ efflux in response to carbamylcholine; (3) re-entry of K+ into cells after carbamylcholine stimulation; (4) the maintenance of K+ in the absence of added effectors. No differences were observed between cells pretreated with serum and cells to which serum was added at the time of cholinergic stimulation of secretion. At high concentrations, serum from both normal individuals and those with cystic fibrosis severely damaged the cells in a rapid and irreversible fashion. K+ transport has a direct, closely coupled, specific relationship to secretory activity and is Ca++ dependent in this system. From these observations, we conclude that serum from individuals with cystic fibrosis does not contain chemical compounds which specifically alter the major components of ion-coupling to secretion or the secretory process itself in salivary gland acinar cells.
为了证明影响离子偶联分泌的因素的存在，研究了囊性纤维化患者血清对大鼠下颌下腺（一种浆液性唾液腺）分散腺泡细胞内钾离子的维持、外流和摄取的作用。在钾离子转运的以下参数上未显示出对囊性纤维化血清有特异性影响：（1）胆碱能激动剂氨甲酰胆碱对钾离子外流的最大刺激作用；（2）氨甲酰胆碱刺激下钾离子外流的剂量反应曲线；（3）氨甲酰胆碱刺激后钾离子重新进入细胞；（4）在无添加效应物情况下钾离子的维持。在用血清预处理的细胞和在胆碱能刺激分泌时添加血清的细胞之间未观察到差异。在高浓度下，正常个体和囊性纤维化患者的血清均以快速且不可逆的方式严重损伤细胞。在该系统中，钾离子转运与分泌活性具有直接、紧密偶联的特定关系，且依赖于钙离子。基于这些观察结果，我们得出结论，囊性纤维化患者的血清中不含有能特异性改变唾液腺腺泡细胞中离子偶联分泌的主要成分或分泌过程本身的化合物。
