Quissell D O, McDonald R J, Barzen K A, Deisher L M
Pediatr Res. 1983 Nov;17(11):899-902. doi: 10.1203/00006450-198311000-00012.
The action of serum from cystic fibrosis patients, obligatory heterozygotes, siblings, and normal patients on mucin secretion from dispersed rat submandibular cells was investigated in an attempt to demonstrate the existence of cystic fibrosis specific factors affecting mucin secretion. No effects specific to cystic fibrosis serum were demonstrated using the following parameters for evaluation: (1) maximal stimulation of mucin release by a beta-adrenergic agonist, (-)-isoproterenol; (2) basal release (unstimulated secretion) of mucin material; and (3) the dose-response relationship for mucin release after beta-adrenergic receptor stimulation. No differences were observed in mucin secretion using media concentrations of serum of up to 10%. From these data, we conclude that serum from individuals with cystic fibrosis does not contain bioactive factors at concentrations that specifically alter mucin secretion in rat submandibular acinar cells.
研究了囊性纤维化患者、 obligatory杂合子、同胞以及正常患者的血清对分散的大鼠下颌下腺细胞粘蛋白分泌的作用，以试图证明存在影响粘蛋白分泌的囊性纤维化特异性因子。使用以下评估参数未证明囊性纤维化血清有特异性作用：（1）β-肾上腺素能激动剂（-）-异丙肾上腺素对粘蛋白释放的最大刺激；（2）粘蛋白物质的基础释放（未刺激的分泌）；以及（3）β-肾上腺素能受体刺激后粘蛋白释放的剂量反应关系。使用高达10%的血清培养基浓度时，未观察到粘蛋白分泌有差异。根据这些数据，我们得出结论，囊性纤维化患者的血清中不存在能特异性改变大鼠下颌下腺腺泡细胞粘蛋白分泌的浓度的生物活性因子。
