Durie B G, Salmon S E
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The application of the tumor stem cell assay to the study of human tumor cell kinetics has the potential for major advances in our knowledge of proliferative characteristics of clonogenic human tumor cells. From simultaneous evaluation of in vitro drug sensitivity, in vitro doubling time, and the thymidine suicide index, plus flow cytometry, cytogenetic analysis, and assessment of differentiation markers, substantial insights into the basic biology of tumor cell growth may be attained. As discussed in other chapters, using modifications of the two-layer system, one can assess both local cell-mediated and humoral factors that might influence in vitro kinetics and drug sensitivity. The next few years should see the acquisition and integration of valuable new information that should allow more rational approaches to the treatment of tumors by taking full advantage of information on both kinetics and drug sensitivity of clonogenic human tumor cells.
将肿瘤干细胞检测应用于人类肿瘤细胞动力学研究，有望在我们对克隆源性人类肿瘤细胞增殖特性的认识上取得重大进展。通过同时评估体外药物敏感性、体外倍增时间和胸腺嘧啶核苷自杀指数，再加上流式细胞术、细胞遗传学分析以及分化标志物评估，或许能深入了解肿瘤细胞生长的基础生物学特性。如其他章节所讨论的，通过对双层系统进行改进，人们可以评估可能影响体外动力学和药物敏感性的局部细胞介导因素和体液因素。在接下来的几年里，有望获取并整合有价值的新信息，从而能够通过充分利用克隆源性人类肿瘤细胞动力学和药物敏感性方面的信息，采取更合理的肿瘤治疗方法。
