Copeland M, Lippiello L, Steensland G, Guralnick W C, Mankin H J
Prostaglandins. 1980 Dec;20(6):1075-87. doi: 10.1016/0090-6980(80)90061-1.
Suspensions of aggregated chondrocytes display active prostaglandin (PG) production. Radioimmunoassay of culture media and thin layer chromatographic analysis suggests that PGE2 is the primary PG synthesized. In order of decreasing concentration, the following PG were tentatively identified; PGE greater than PGI greater than PGA + PGB greater than or equal to PGF1+2 greater than TxB. An inverse logarithmic relationship was identified between PG synthesis and cells cultured at densities of 1.5 to 7.5 x 10(6) cells/ml. Little or no change in the PG distribution profile was seen at these high cell densities. Maximum PG synthesis was attained after 36 hours of incubation with persistence of high synthetic levels up to 48 hours. PGE2 production measured at various post-isolation intervals indicated an initial high rate of synthesis during the first 4 hours which decreased with time up to 24 hours. Cartilage explant organ cultures demonstrated a similar level of PG synthesis suggesting minimal effect of matrix on cellular PG production. Indomethacin (5 microgram/ml) inhibited PG synthesis by 70% within 4 hours and 85% after 24 hours of exposure. Arachidonic acid supplementation (10 microM) stimulated PG synthesis by 300%.
聚集软骨细胞悬液显示出活跃的前列腺素（PG）产生。对培养基的放射免疫分析和薄层色谱分析表明，PGE2是合成的主要前列腺素。按浓度递减顺序，初步鉴定出以下前列腺素：PGE＞PGI＞PGA + PGB≥PGF1+2＞TxB。在细胞密度为1.5至7.5×10⁶个细胞/毫升的条件下培养细胞时，发现PG合成与细胞之间存在对数反比关系。在这些高细胞密度下，PG分布谱几乎没有变化。孵育36小时后达到最大PG合成，直至48小时仍保持高合成水平。在分离后的不同时间间隔测量PGE2产生量，结果表明在最初4小时内合成速率较高，随后至24小时随时间下降。软骨外植体器官培养显示出类似水平的PG合成，表明基质对细胞PG产生的影响最小。吲哚美辛（5微克/毫升）在4小时内抑制PG合成70%，暴露24小时后抑制85%。补充花生四烯酸（10微摩尔）可使PG合成增加300%。
