Pearson H, Arthur G H, Rosevink B, Kakati B
Vet Rec. 1980 Sep 20;107(12):285-7. doi: 10.1136/vr.107.12.285.
In an attempt to induce permanent aspermia the caudae epididymes of 17 bulls were subjected to ligation or injection with ethanolamine or chlorhexidine in dimethyl sulphoxide. Nylon and polyglycolic acid ligatures had little or no effect on the sperm counts although these materials were used in only three bulls. Steel ligation induced aspermia of several weeks' duration in four of six bulls. Although the effect in at least three of these animals might have been permanent, the trial was discontinued because the other two bulls, treated identically, showed little response. Ethanolamine injected into the epididymis also had little or no effect on the ejaculate. By contrast the chlorhexidine preparation produced an almost immediate aspermia sustained until the bulls were slaughtered a year later.
为了诱导永久性无精子症，对17头公牛的附睾尾部进行结扎，或注射二甲基亚砜中的乙醇胺或氯己定。尼龙和聚乙醇酸结扎线对精子计数几乎没有影响，尽管仅在三头公牛中使用了这些材料。金属结扎使六头公牛中的四头出现了持续数周的无精子症。尽管其中至少三头动物的这种影响可能是永久性的，但试验中断了，因为另外两头接受相同处理的公牛几乎没有反应。向附睾注射乙醇胺对射精量也几乎没有影响。相比之下，氯己定制剂几乎立即产生了无精子症，并一直持续到一年后公牛被屠宰。
