一种用于恢复经间接免疫荧光技术处理的褪色组织标本荧光的复染方法。
A restaining method to restore fluorescence in faded preparations of tissues treated with the indirect immunofluorescence technique.
作者信息
Weinstein W M, Lechago J
出版信息
J Immunol Methods. 1977;17(3-4):375-8. doi: 10.1016/0022-1759(77)90121-1.
PMID:72120
Abstract
We report a restainin method for restoring fluorescence in paraffin-embedded tissue sections previously treated with the indirect immunofluorescence technique. Antisera to gastrin and group II pepsinogens were used. Fluorescence was restored in completely faded sections retrived from storage files, as well as in sections that had faded partially either with exposure to fluorescence microscope illumination or after counterstaining with hematoxylin and eosin.
摘要
我们报告了一种用于在先前用间接免疫荧光技术处理过的石蜡包埋组织切片中恢复荧光的再染色方法。使用了针对胃泌素和II组胃蛋白酶原的抗血清。在从储存档案中取出的完全褪色的切片中，以及在因暴露于荧光显微镜照明或用苏木精和伊红复染后部分褪色的切片中，荧光都得以恢复。
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