Kaplan M S
Anat Rec. 1980 Aug;197(4):495-502. doi: 10.1002/ar.1091970411.
An adult rhesus monkey was injected intraperitoneally with [H3] thymidine (2.3 microCi/gram body weight) and perfused 90 minutes later with a mixture of aldehydes. One and a half micrometer plastic sections were then cut and dipped into liquid emulsion for radioautography. Labeled cells were observed in the choroid plexus of the anterior lateral ventricle; cell identification was evaluated using electron micrographs taken from serial thin sections of re-embedded. radioautographic 1.5-micron sections. The ultrastructure and location of both mitotic figures and labeled cells confirmed the presence of undifferentiated basal choroid plexus epithelial cells in the adult primate central nervous system.
给一只成年恒河猴腹腔注射[H3]胸苷(2.3微居里/克体重),90分钟后用醛类混合物灌注。然后切取1.5微米的塑料切片,浸入液体乳剂中进行放射自显影。在前外侧脑室脉络丛中观察到标记细胞;使用从重新包埋的放射自显影1.5微米切片的连续薄切片拍摄的电子显微照片评估细胞鉴定。有丝分裂图和标记细胞的超微结构及位置证实了成年灵长类中枢神经系统中存在未分化的基底脉络丛上皮细胞。