Wilkie B N, Markham R J, Shewen P E
Am J Vet Res. 1980 Nov;41(11):1773-8.
In 3 experiments, calves were vaccinated by intrabronchial or subcutaneous injection of formalinized Pasteurella haemolytica. Immunization was evaluated by assessing the clinical and pathologic responses to pulmonary challenge exposure with live homologous bacteria. Serum and bronchoalveolar washings were titrated for antibody by indirect (antiglobulin) bacterial agglutination. Response to challenge exposure was determined by increased rectal temperature and faster respiratory rate. In all experiments, the most obvious clinical change occurred in challenge-exposed calves which had been vaccinated by the subcutaneous injection of killed P haemolytica with added adjuvant. Also, macroscopic pulmonic lesions were recorded most frequently in calves vaccinated by this route. In all instances, immunization resulted in elevated anti-P haemolytica titers both in lung and serum.
在3项实验中，通过支气管内或皮下注射甲醛化溶血巴斯德氏菌对犊牛进行疫苗接种。通过评估对用活的同源细菌进行肺部激发暴露的临床和病理反应来评价免疫效果。通过间接（抗球蛋白）细菌凝集滴定血清和支气管肺泡灌洗液中的抗体。通过直肠温度升高和呼吸频率加快来确定对激发暴露的反应。在所有实验中，最明显的临床变化发生在用添加佐剂的灭活溶血巴斯德氏菌皮下注射进行疫苗接种的激发暴露犊牛身上。同样，通过该途径接种疫苗的犊牛中，肺部宏观病变记录最为频繁。在所有情况下，免疫均导致肺和血清中抗溶血巴斯德氏菌滴度升高。
