Taboada J, González-Diddi M
Arch Invest Med (Mex). 1980;11(4):435-43.
The cytotoxic effects of sesquiterpene gamma-lactones in tissue culture were studied on two different cell lines, one of them fibroblastoid cells L-929 from mice areolar tissue, and the other epithelial cells HEp-2 from human laryngeal carcinoma. Ten lactones were tested, all of them having in their molecule an alpha methylene gamma lactone group, which had been described as conferring cytotoxic activity. Five of these lactones have a furanic ring in their molecule whose presence gives them a higher cytotoxic activity. Different doses of each lactone were tested. Cell number and exclusion tests were simultaneously performed per dose, obtaining for all of them the ED50, which was the calculated as the effective dose inhibiting growth to 50 per cent of control growth. The ED50 was about 1 to 5 microgram in those lactones with furanic ring. The two cell lines had a different response to the same lactone.
研究了倍半萜γ-内酯在组织培养中对两种不同细胞系的细胞毒性作用，其中一种是来自小鼠乳晕组织的成纤维样细胞L-929，另一种是来自人喉癌的上皮细胞HEp-2。测试了10种内酯，它们分子中均含有α-亚甲基γ-内酯基团，该基团已被描述为具有细胞毒性活性。其中5种内酯分子中含有呋喃环，这使其具有更高的细胞毒性活性。对每种内酯测试了不同剂量。每剂量同时进行细胞计数和排斥试验，计算出所有内酯的半数有效剂量（ED50），即抑制生长至对照生长50%的有效剂量。含有呋喃环的内酯的ED50约为1至5微克。两种细胞系对同一种内酯的反应不同。
