未处理及苯巴比妥处理的大鼠肝脏粗面和滑面内质网膜中的磷脂不对称性
Phospholipid asymmetry in rough- and smooth-endoplasmic-reticulum membranes of untreated and phenobarbital-treated rat liver.
作者信息
Bollen I C, Higgins J A
出版信息
Biochem J. 1980 Sep 1;189(3):475-80. doi: 10.1042/bj1890475.
Abstract
Phospholipase C was used as a probe for the distribution of phospholipids about the membrane of rough and smooth microsomal fractions from normal and phenobarbital-treated rat liver. All membranes exhibited an asymmetric distribution, with phosphatidylethanolamine and phosphatidylserine concentrated in the inner leaflet of the bilayer and phosphatidylcholine and sphingomyelin concentrated in the outer leaflet. The only phospholipid showing a significant difference in distribution between fractions was phosphatidylcholine, which was shifted towards the outer leaflet in the smooth microsomal fraction compared with the rough microsomal fraction, and towards the outer leaflet in both rough and smooth microsomal fractions from phenobarbital-treated liver compared with the same preparations from untreated rat liver. Apart from this small change, the asymmetric distribution of phospholipids was conserved in microsomal fractions which had proliferated in response to phenobarbital and in which the protein composition had changed.
摘要
磷脂酶C被用作一种探针，以研究正常大鼠肝脏和经苯巴比妥处理的大鼠肝脏的粗糙和光滑微粒体部分膜上磷脂的分布情况。所有膜均呈现不对称分布，磷脂酰乙醇胺和磷脂酰丝氨酸集中在双层膜的内小叶，而磷脂酰胆碱和鞘磷脂集中在外小叶。各部分之间在分布上显示出显著差异的唯一磷脂是磷脂酰胆碱，与粗糙微粒体部分相比，它在光滑微粒体部分中向外小叶转移，并且与未经处理的大鼠肝脏的相同制剂相比，在经苯巴比妥处理的肝脏的粗糙和光滑微粒体部分中均向外小叶转移。除了这一微小变化外，磷脂的不对称分布在因苯巴比妥而增殖且蛋白质组成发生变化的微粒体部分中得以保留。
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