Ting-Beall H P, Costello M J, Shoemaker D, Holland V F
Biochim Biophys Acta. 1981 Feb 6;640(3):807-11. doi: 10.1016/0005-2736(81)90111-5.
Both negative-stain and freeze-fracture electron microscopic techniques revealed that the ultrastructure of resealed white ghosts prepared at high dilution during the hemolysis step is very different from that of resealed ghosts prepared at low or moderate dilution (pink ghosts). The negative-stained resealed white ghosts showed light halo substructures on membrane surfaces and protrusions at the edge of the ghosts. Freeze-fracturing of these ghosts showed that membrane blebbing had occurred and that fragments of the membranes resealed to form small right-side-out vesicles ranging from 0.1 to 0.3 micrometer in diameter.
负染色和冷冻断裂电子显微镜技术均显示，在溶血步骤中以高稀释度制备的重封白血影的超微结构与以低或中等稀释度制备的重封血影（粉红血影）有很大不同。负染色的重封白血影在膜表面显示出光晕亚结构，且在血影边缘有突起。这些血影的冷冻断裂显示发生了膜泡形成，并且重新封闭的膜片段形成了直径为0.1至0.3微米的小外翻囊泡。
