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[封闭及酞菁染料吸附过程中白色红细胞影的形态和超微结构变化]

[Changes in the form and ultrastructure of white erythrocyte ghosts during sealing and phthalocyanine dye sorption].

作者信息

Brudnaia M S, Kirpichnikova K M, Komissarchuk Ia Iu, Levin S V

出版信息

Tsitologiia. 1984 Feb;26(2):184-7.

PMID:6202029
Abstract

A study was made of the shape and ultrastructure of human erythrocytes unsealed and resealed for sucrose. The unsealed white ghosts assume unregular ameboid-like shapes. After resealing they acquire more regular spherical forms; the sub-membrane layer is dissociated, the plasma membrane undergoes numerous deformations. The same ultrastructure is revealed for the ghosts obtained from erythrocytes stained with phthalocyanine anionic dye (Heliogen Blue SBL). This dye is bound to the outer surface of the red blood cell, occupying no more than 2-4% of the cell surface.

摘要

对用于蔗糖的人红细胞去密封和重新密封后的形状及超微结构进行了研究。去密封的白色血影呈现不规则的类阿米巴样形状。重新密封后,它们获得更规则的球形;膜下层解离,质膜经历多次变形。从用酞菁阴离子染料(日光蓝SBL)染色的红细胞获得的血影也呈现相同的超微结构。这种染料结合在红细胞的外表面,占据不超过细胞表面的2 - 4%。

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