Frolik C A, Dart L L, Sporn M B
Biochim Biophys Acta. 1981 Jan 26;663(1):329-35. doi: 10.1016/0005-2760(81)90218-6.
All-trans-[3H]retinyl acetate has been shown to be metabolized to all-trans-[3H]retinoic acid in a target tissue of vitamin A action, the hamster trachea in organ culture. That the compound produced is indeed all-trans-retinoic acid is demonstrated by chromatography of the biosynthetically produced retinoic acid with synthetic all-trans-retinoic acid in two different high-pressure liquid chromatographic systems, either as the free acids in a reverse-phase system or as the methyl esters in a normal-phase system. The all-trans-[3H]retinoic acid was also found in the tracheal epithelium and cartilage as well as in the medium. In addition the tracheal tissue also contained retinyl palmitate and other esters. Finally, further in vitro metabolism of [3H]retinyl acetate paralleled the metabolism of [14C]retinoic acid suggesting that these two compounds are being metabolized through similar pathways.
全反式-[3H]醋酸视黄酯已被证明在维生素A作用的靶组织（器官培养中的仓鼠气管）中代谢为全反式-[3H]视黄酸。通过在两种不同的高压液相色谱系统中，将生物合成产生的视黄酸与合成的全反式视黄酸进行色谱分析，无论是在反相系统中作为游离酸，还是在正相系统中作为甲酯，都证明所产生的化合物确实是全反式视黄酸。在气管上皮、软骨以及培养基中也发现了全反式-[3H]视黄酸。此外，气管组织还含有棕榈酸视黄酯和其他酯类。最后，[3H]醋酸视黄酯的进一步体外代谢与[14C]视黄酸的代谢相似，这表明这两种化合物是通过相似的途径进行代谢的。
