Grumbkova L O, Bokhon'ko A I
Biokhimiia. 1980 Jun;45(6):1146-50.
Two preparations of chromatin were isolated frm two divisions of rat brain differing in the average content of RNA in the cells and in the activities of nuclear forms of RNA-polymerases. Cerebellar chromatin was shown to contain far fewer non-histone proteins than the chromatin from the large hemispheres. The template properties of chromatin preparations were studied in a RNA synthesis system by two different methods, using bacterial RNA-polymerase. No differences in the template activity of the two preparations were revealed. It was assumed that the rate of RNA synthesis in these two divisions of rat brain are predominantly due to the activity of nuclear RNA-polymerases rather than to the state of the corresponding matrix.
从大鼠脑的两个不同部位分离出两种染色质制剂,这两个部位的细胞中RNA平均含量以及核形式的RNA聚合酶活性存在差异。结果表明,小脑染色质所含的非组蛋白比大脑半球的染色质少得多。利用细菌RNA聚合酶,通过两种不同方法在RNA合成系统中研究了染色质制剂的模板特性。未发现两种制剂的模板活性存在差异。据推测,大鼠脑这两个部位的RNA合成速率主要取决于核RNA聚合酶的活性,而非相应模板的状态。