Grumbkova L O, Bokhon'ko A I
Biokhimiia. 1980 Nov;45(11):2013-8.
The cell nuclei of cerebellum and large hemispheres of rat brain were treated with micrococcal nuclease (EC 3.1.4.7); the resulting DNA fragments were separated in 4% polyacrylamide gel. The experimental data are indicative of differences in the structural organization of chromatins from different divisions of the brain: the average value of the repeating DNA sequences of chromatin from the large hemispheres was 184 base pairs against 199 in case of cerebellar chromatin. The DNA fragment incorporated into the nucleosomal nucleus contained 140 base pairs in both chromatin preparations. The dependence of the size of the DNA "bridge" between the vicinal nucleosomal nuclei on the protein composition and template activity of chromatin preparations is discussed.
用微球菌核酸酶（EC 3.1.4.7）处理大鼠脑小脑和大脑半球的细胞核；将得到的DNA片段在4%聚丙烯酰胺凝胶中进行分离。实验数据表明大脑不同区域染色质的结构组织存在差异：大脑半球染色质重复DNA序列的平均值为184个碱基对，而小脑染色质为199个碱基对。在两种染色体制备物中，掺入核小体核的DNA片段均含有140个碱基对。讨论了相邻核小体核之间DNA“桥”的大小对染色体制备物蛋白质组成和模板活性的依赖性。
