Moulinoux J P, Javre J L
Bull Assoc Anat (Nancy). 1980 Sep;64(186):425-30.
If rat liver histological sections, cut by cryostat, incubated in presence of different putrescine concentrations (diluted in Hanks medium) and stained with P.A.S. show no real changes in glycogenolysis, others in a medium containing spermidine or better spermine, proportionally to the concentration in polyamine and duration of incubation, show an increase in the staining of the hepatic cytoplasms compared with the relative controls which are sections incubated in Hanks medium during the same time. So, in these conditions, putrescine doesn't seem to have an influence on glycogenolysis, but spermidine and spermine have a protective effect on hepatic glycogen.
如果用低温恒温器切割的大鼠肝脏组织切片，在不同腐胺浓度（用汉克斯培养基稀释）存在的情况下孵育，并用PAS染色，糖原分解没有真正变化；而在含有亚精胺或更好的精胺的培养基中，与在相同时间内在汉克斯培养基中孵育的相对对照切片相比，根据多胺浓度和孵育时间的比例，肝细胞质染色增加。因此，在这些条件下，腐胺似乎对糖原分解没有影响，但亚精胺和精胺对肝糖原具有保护作用。
