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蝾螈(墨西哥钝口螈)视顶盖中视神经传入突触的识别与形态测量评估。

Identification and morphometric evaluation of the synapses of optic nerve afferents in the optic tectum of the axolotl (Ambystoma mexicanum).

作者信息

Ingham C A, Güldner F H

出版信息

Cell Tissue Res. 1981;214(3):593-611. doi: 10.1007/BF00233499.

DOI:10.1007/BF00233499
PMID:7214469
Abstract

Ther terminals of retinal afferents in the tectum of the axolotl have been identified ultrastructurally using techniques of horseradish peroxidase-filling and degeneration. The mitochondria in filled structures show a characteristic electron-lucent matrix. After both eyes have been removed, terminals with light mitochondria disappear from the area known to receive an optic input. In this area the presence of light mitochondria is almost always diagnostic of the retinal origin of a bouton. The synapses are similar to those assumed to be of retinal origin in other vertebrates. Detailed morphometric analysis has been carried out on identified optic synapses in the optic tectum of the axolotl.

摘要

利用辣根过氧化物酶填充和变性技术,在超微结构水平上鉴定了蝾螈视顶盖中视网膜传入纤维的终末。被填充结构中的线粒体显示出特征性的电子透明基质。双眼摘除后,具有浅色线粒体的终末从已知接受视觉输入的区域消失。在该区域,浅色线粒体的存在几乎总是一个bouton源自视网膜的诊断依据。这些突触与其他脊椎动物中假定源自视网膜的突触相似。已对蝾螈视顶盖中已鉴定的视觉突触进行了详细的形态计量分析。

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The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中,将高pH值的柠檬酸铅用作电子不透明染色剂。
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