Sinha A A, Bentley M D, Pomroy F E, Jamuar M P
Cell Tissue Res. 1981;215(3):547-61. doi: 10.1007/BF00233531.
Ultrastructure of the ventral prostate glands was studied in mice castrated for 1 through 60 days and for 11 and 17 months and in age-matched normals. We have described freeze-fracture and ultrastructural characteristics of acinar epithelial cells in addition to the patterns of thymidine incorporation in the cells of castrates and normal animals. Our study has shown a biphasic pattern of prostatic involution in the long-term castrated mice. In castrates the initial atrophy of prostate glands occurred by sloughing of the apical portions of columnar cells, autophagia of the cytoplasmic organelles as well as by occasional sloughing of the individual cells into the acinar lumen. Concurrent with the initial atrophy, the glands and stroma were infiltrated by neutrophils and lymphocytes. The cell loss by sloughing and leucocyte infiltration of glands became infrequent in 7- to 21-day castrates. However, the cell loss by sloughing increased secondarily in mice castrated for 21 to 37 days along with the increased leucocyte infiltration of the glands. The cell loss became minimal in castrates of 60 days and beyond. Our evidence suggests that the cell loss by sloughing was an active process in the involution of prostate glands which also showed differential sensitivity to castration stimuli in mice.
对阉割1至60天、11个月和17个月的小鼠以及年龄匹配的正常小鼠的腹侧前列腺进行了超微结构研究。除了描述去势动物和正常动物细胞中胸腺嘧啶核苷掺入模式外，我们还描述了腺泡上皮细胞的冷冻断裂和超微结构特征。我们的研究显示，长期去势小鼠的前列腺退化呈双相模式。在去势小鼠中，前列腺最初的萎缩是通过柱状细胞顶端部分的脱落、细胞质细胞器的自噬以及偶尔单个细胞脱落到腺泡腔内而发生的。与最初的萎缩同时发生的是，腺体和基质被中性粒细胞和淋巴细胞浸润。在去势7至21天的小鼠中，通过脱落导致的细胞丢失和腺体的白细胞浸润变得不常见。然而，在去势21至37天的小鼠中，随着腺体白细胞浸润的增加，通过脱落导致的细胞丢失再次增加。在60天及以上的去势小鼠中，细胞丢失变得最小。我们的证据表明，通过脱落导致的细胞丢失是前列腺退化过程中的一个活跃过程，在小鼠中对去势刺激也表现出不同的敏感性。
