Yamada N, Murase T, Akanuma Y, Itakura H, Kosaka K
Clin Chim Acta. 1981 Feb 19;110(1):45-9. doi: 10.1016/0009-8981(81)90298-9.
A patient with nephrotic syndrome and morbus Kimura (eosinophilic granuloma) showed chylous ascites. Ascites chylomicrons were analyzed and used to study the substrate specificity of lipoprotein lipase and hepatic triglyceride lipase. Ascites triglyceride and cholesterol concentrations were 191 and 12 mg/dl, respectively. Both apo CII and apo CIII content in ascites were approximately one-third of those of plasma from normal subjects. Ascites chylomicrons were incubated with either lipoprotein lipase or hepatic triglyceride lipase, which were prepared from postheparin plasma using heparin-Sepharose affinity chromatography. Lipoprotein lipase hydrolyzed ascites chylomicrons, while hepatic triglyceride lipase did not. These results suggest different functions of these two lipases in chylomicron catabolism.
一名患有肾病综合征和木村病（嗜酸性肉芽肿）的患者出现了乳糜性腹水。对腹水中的乳糜微粒进行了分析，并用于研究脂蛋白脂肪酶和肝甘油三酯脂肪酶的底物特异性。腹水甘油三酯和胆固醇浓度分别为191和12mg/dl。腹水中载脂蛋白CII和载脂蛋白CIII的含量均约为正常受试者血浆的三分之一。将腹水乳糜微粒与脂蛋白脂肪酶或肝甘油三酯脂肪酶一起孵育，这两种酶是使用肝素-琼脂糖亲和色谱法从肝素后血浆中制备的。脂蛋白脂肪酶可水解腹水乳糜微粒，而肝甘油三酯脂肪酶则不能。这些结果表明这两种脂肪酶在乳糜微粒分解代谢中具有不同的功能。
