Scott J N, Weber D F
J Morphol. 1977 Oct;154(1):133-45. doi: 10.1002/jmor.1051540109.
The juction between human primary dentine and regular and irregular secondary dentine was examined with a number of different light and electron microscopic techniques. In decalcified material, a narrow band along the innermost surface of the primary dentine stained intensely. The walls of the tubules within the band stained intensely, whereas the tubular walls within the bulk of the primary dentine were not stained. Generally, the walls of the tubules in both types of secondary dentine were also preferentially stained. Although not readily apparent in ground sections, observations of thin sections revealed a dramatic reduction in the number of tubules in regular secondary dentine. Generally, the radiodensity of the intertubular matrix was the same in primary and secondary dentine and the intensely stained band was not seen radiographically. The pulpal ends of the tubules in primary dentine were often occluded with a material having the same radiodensity as peritubular matrix. Both patent and occluded tubules were seen in irregular secondary dentine. Scanning electron microscopy of acid-etched specimens of secondary dentine revealed that some tubules had irregular walls of highly mineralized matrix which was less acid-soluble then the peritubular matrix of primary dentine.
运用多种不同的光学和电子显微镜技术，对人类原发性牙本质与规则及不规则继发性牙本质之间的交界处进行了检查。在脱钙材料中，沿着原发性牙本质最内表面有一条窄带被强烈染色。该带内的小管壁被强烈染色，而原发性牙本质主体内的小管壁未被染色。一般来说，两种类型继发性牙本质中的小管壁也都被优先染色。尽管在磨片上不太明显，但对薄片的观察显示规则继发性牙本质中的小管数量显著减少。一般来说，原发性和继发性牙本质中管间基质的放射密度相同，且在射线照片上未见到强烈染色带。原发性牙本质中小管的牙髓端常被与管周基质具有相同放射密度的物质堵塞。在不规则继发性牙本质中可见开放和堵塞的小管。对继发性牙本质酸蚀标本的扫描电子显微镜观察显示，一些小管具有高度矿化基质的不规则壁，其酸溶性低于原发性牙本质的管周基质。
