Malan S, Lewis R A
In Vitro. 1980 Dec;16(12):1092-8. doi: 10.1007/BF02619260.
Porcine skin nucleoplasmic extract (PSNE) was shown to alter the incorporation of [3H]thymidine into DNA of selected porcine, bovine, and human cell populations in culture. PSNE stimulated incorporation of [3H]thymidine into DNA of porcine and bovine dermal cells an average of 300 and 200% of control value, respectively. When porcine and bovine epidermal cells were exposed to PSNE the treatment inhibited [3H]thymidine incorporation into DNA by an average of 48 and 45%, respectively. Similar inhibitions were observed for porcine and bovine kidney, porcine lung, and human KB cells. Thus, the effect of PSNE on the incorporation of [3H]thymidine into DNA of various cultured cells was either stimulatory to dermal cells or inhibitory to a variety of other cell types, including skin epidermal cells. The stimulatory and inhibitory effects of PSNE were abolished by heating PSNE for 5 min in boiling water before its addition to cell cultures. This suggests that macromolecular structure is important in the action of PSNE.
猪皮肤核质提取物（PSNE）被证明可改变[3H]胸苷掺入培养的特定猪、牛和人类细胞群体DNA中的情况。PSNE刺激[3H]胸苷掺入猪和牛真皮细胞DNA中的量分别平均为对照值的300%和200%。当猪和牛表皮细胞暴露于PSNE时，该处理分别平均抑制[3H]胸苷掺入DNA量的48%和45%。在猪和牛肾脏、猪肺以及人类KB细胞中也观察到了类似的抑制作用。因此，PSNE对[3H]胸苷掺入各种培养细胞DNA的影响，要么对真皮细胞有刺激作用，要么对包括皮肤表皮细胞在内的多种其他细胞类型有抑制作用。在将PSNE添加到细胞培养物之前，将其在沸水中加热5分钟，PSNE的刺激和抑制作用就会消失。这表明大分子结构在PSNE的作用中很重要。
