小鼠生殖道的分子分化：用于形态学和生化相关性研究的无血清器官培养系统
Molecular differentiation of the mouse genital tract: serum-free organ culture system for morphological and biochemical correlations.
作者信息
Newbold R R, Carter D B, Harris S E, McLachlan J A
出版信息
In Vitro. 1981 Jan;17(1):51-4. doi: 10.1007/BF02618030.
PMID:7216239
Abstract
This report describes a serum-free system for studying the fetal mouse genital tract in organ culture. Using the techniques of isoelectric focusing and gel electrophoresis of polypeptides synthesized by the organ explant in culture, the biochemical integrity of as little as 1 to 2 mg of this tissue was determined during the period of culture. Thus, differentiation of the fetal genital tissue in organ culture can be assessed by both morphological and biochemical criteria.
摘要
本报告描述了一种用于在器官培养中研究胎鼠生殖道的无血清系统。利用等电聚焦技术和培养的器官外植体合成的多肽的凝胶电泳技术，在培养期间测定了低至1至2毫克这种组织的生化完整性。因此，可以通过形态学和生化标准评估器官培养中胎儿生殖组织的分化情况。
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