Tveit K M, Fodstad O, Olsnes S, Pihl A
Int J Cancer. 1980 Dec 15;26(6):717-22. doi: 10.1002/ijc.2910260604.
Single-cell suspensions prepared from five human melanomas, grown serially as xenografts in athymic nude mice, were exposed in vitro to increasing concentrations of DTIC (Dacarbazine), CCNU (Lomustine), procarbazine, vinblastine, and the cancerostatic lectins abrin and ricin. The in vitro chemosensitivity of the cells, as measured by the drug concentrations required to inhibit colony formation in soft agar by 50%, was correlated with the growth delay of the xenografts in vivo, previously observed after treatment of the animals with maximum tolerable doses of the same drugs. It was found that for each drug the in vitro sensitivity of the different xenografts was strongly correlated with their response in vivo. The results provide evidence that the soft agar test, as carried out here, adequately reflects the relative sensitivity of the xenografts in vivo. The data indicate that human xenografts may be used to develop quantitative in vitro chemosensitivity tests.
从五只人黑色素瘤中制备的单细胞悬液，这些黑色素瘤在无胸腺裸鼠体内作为异种移植物连续传代生长，将其在体外暴露于浓度不断增加的达卡巴嗪（DTIC）、洛莫司汀（CCNU）、丙卡巴肼、长春碱以及抗癌凝集素相思子毒素和蓖麻毒素中。通过抑制软琼脂中集落形成50%所需的药物浓度来衡量细胞的体外化学敏感性，该敏感性与之前用相同药物的最大耐受剂量处理动物后观察到的异种移植物在体内的生长延迟相关。结果发现，对于每种药物，不同异种移植物的体外敏感性与其在体内的反应密切相关。这些结果证明，此处进行的软琼脂试验充分反映了异种移植物在体内的相对敏感性。数据表明，人异种移植物可用于开发定量体外化学敏感性试验。
