用碳-13核磁共振研究神经母细胞瘤x胶质瘤杂交细胞对脑啡肽和脑啡肽酰胺的降解作用

Degradation of enkephalin and enkephalinamide by neuroblastoma x glioma hybrid cells as studied by 13C n.m.r.

作者信息

Deslauriers R, Jarrell H C, Griffith D W, McGregor W H, Smith I C

出版信息

Int J Pept Protein Res. 1980 Nov;16(5):487-93. doi: 10.1111/j.1399-3011.1980.tb02974.x.

DOI:10.1111/j.1399-3011.1980.tb02974.x

Abstract

13C n.m.r. and thin-layer chromatography were used to monitor the degradation of methionine-enkephalinamide in the presence of neuroblastoma x glioma hybrid cells (NG 108-15) and membranes. Puromycin and trypsin treatment failed to protect enkephalinamide from degradation over long periods of time (up to 24 hours). The major degradation products of [3[2-13C]glycine] methionine-enkephalinamide observed by 13 C n.m.r. showed glycine-3 in a non-terminal position, an N-terminal position and free glycine. A minor component showed glycine-3 in a C-terminal position.

摘要

采用13C核磁共振和薄层色谱法监测甲硫氨酸脑啡肽酰胺在神经母细胞瘤x胶质瘤杂交细胞（NG 108 - 15）及细胞膜存在的情况下的降解情况。嘌呤霉素和胰蛋白酶处理在长时间（长达24小时）内未能保护脑啡肽酰胺不被降解。通过13C核磁共振观察到的[3[2 - 13C]甘氨酸]甲硫氨酸脑啡肽酰胺的主要降解产物显示，甘氨酸-3处于非末端位置、N末端位置以及游离甘氨酸状态。一个次要成分显示甘氨酸-3处于C末端位置。