用单体pVA736质粒脱氧核糖核酸转化血链球菌
Transformation of Streptococcus sanguis with monomeric pVA736 plasmid deoxyribonucleic acid.
作者信息
Macrina F L, Jones K R, Welch R A
出版信息
J Bacteriol. 1981 May;146(2):826-30. doi: 10.1128/jb.146.2.826-830.1981.
Abstract
Monomeric and oligomeric forms of a 5.0 x 10(6)-dalton plasmid (conferring erythromycin resistance) were able to genetically transform naturally competent Streptococcus sanguis. Transformation with electrophoretically purified monomer was a second-order process, whereas transformation with a dye-buoyant density gradient-purified plasmid preparation followed one-hit kinetics.
摘要
一个5.0×10⁶道尔顿的质粒（赋予红霉素抗性）的单体和寡聚体形式能够对天然感受态血链球菌进行遗传转化。用电泳纯化的单体进行转化是一个二级过程，而用染料浮力密度梯度纯化的质粒制剂进行转化遵循单次打击动力学。
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