Fekete J, del Castilho P, Kraak J C
J Chromatogr. 1981 Jan 16;204:319-27. doi: 10.1016/s0021-9673(00)81674-x.
Reversed-phase liquid chromatography on a C18 bonded silica with water-alcohol mixtures containing decylamine as the mobile phase was investigated for its applicability to the separation of chlorpromazine, imipramine and their metabolites. The addition of decylamine to the mobile phase was found to be necessary in order to obtain symmetrical peaks. The influence of the decylamine and ethanol concentrations and of the pH of the mobile phase was investigated and these parameters were found to be useful for the adjustment of the retention. The phase system developed was found to be suitable for the required separation. The phase system was applied to the analysis of imipramine and its metabolites, desmethylimipramine and didesmethylimipramine, and desmethylchlorpromazine in human plasma.
研究了以含癸胺的水 - 醇混合物为流动相，在C18键合硅胶上进行反相液相色谱法，用于分离氯丙嗪、丙咪嗪及其代谢物的适用性。发现向流动相中添加癸胺对于获得对称峰是必要的。研究了癸胺和乙醇浓度以及流动相pH值的影响，发现这些参数对于调整保留时间很有用。所开发的相系统被发现适用于所需的分离。该相系统应用于分析人血浆中的丙咪嗪及其代谢物去甲丙咪嗪、双去甲丙咪嗪和去甲氯丙嗪。
