Bredesen J E, Ellingsen O F, Karlsen J
J Chromatogr. 1981 Jan 16;204:361-7. doi: 10.1016/s0021-9673(00)81680-5.
A rapid and simple gas--liquid chromatographic method suitable for routine use is described for quantitation of tricyclic antidepressants. The method can be used for quantitation of amitriptyline, nortriptyline, imipramine, desmethylimipramine, doxepin, desmethyldoxepin, clomipramine, desmethylclomipramine, trimipramine, desmethyltrimipramine and dibenzepine. The method involves one extraction with hexane--isopropyl alcohol, no derivative formation of the metabolites and chromatography using an OV-25 liquid phase which permits isothermal quantitation of the different drugs within 5--10 min. Standard curves for the different tricyclic antidepressants were linear over the concentration range tested (10--1500 micrograms/l) and the maximum coefficient of variation was 7.7%.
本文描述了一种适用于常规使用的快速、简单的气-液色谱法，用于定量三环类抗抑郁药。该方法可用于定量阿米替林、去甲替林、丙咪嗪、去甲丙咪嗪、多塞平、去甲多塞平、氯米帕明、去甲氯米帕明、曲米帕明、去甲曲米帕明和二苯并氮䓬。该方法包括用己烷-异丙醇进行一次萃取，代谢物不形成衍生物，并使用OV-25液相进行色谱分析，可在5-10分钟内对不同药物进行等温定量。不同三环类抗抑郁药的标准曲线在测试浓度范围（10-1500微克/升）内呈线性，最大变异系数为7.7%。
