Bernardis L L, Bellinger L L
J Nutr. 1981 Apr;111(4):721-32. doi: 10.1093/jn/111.4.721.
Young-mature male Sprague-Dawley rats received bilateral lesions in the dorsomedial hypothalamic nuclei (DMNL); sham-operated rats served as controls. After 30 days of a pelleted commercial laboratory diet, they received a powdered lab diet for 10 days. Both groups of rats reduced food intake for the duration of the test. Thus, although grossly hypophagic under ad libitum feeding conditions, DMNL do not impair adaptation to a diet of different consistency. Both DMNL and controls were significantly hypophagic when fed a quinine-adulterated powdered diet, but only on the first of a 9-day regimen. Thus, they show only transitory finickiness. During a 16-day self-selection test with three equicaloric diets with different amounts of the three macronutrients in each (HCD: high carbohydrate, HFD: high fat, and HPD: high protein diet), DMNL rats ate in absolute terms (g/day) the same amount of HCD, significantly less HFD and during two of seven measuring periods, significantly less HPD. However, their intake in percent of total consumption was similar to that of the controls., After a 48-hour fast, DMNL rats lost and regained body though these rats showed hypophagia during the 48-hour realimentation period, their efficiency of food utilization was similar to the controls. Thus, DMNL rats have not lost the capacity to regulate body weight and food intake after fast-induced weight loss. During eight tail pinch (TP) sessions (11 days), DMNL rats were hyperphagic and preferred HCD and HFD in comparison with controls. In their home cages (lab diet) DMNL rats were hypophagic and over 11 days showed a decline in food intake while the controls displayed an increase. In contrast to previous findings, the challenges of the present study unmasked some deficits in energy homeostasis in the DMNL rat.
年轻成年雄性斯普拉格-道利大鼠接受双侧下丘脑背内侧核（DMNL）损伤；假手术大鼠作为对照。在给予颗粒状商业实验室饲料30天后，它们接受粉末状实验室饲料10天。两组大鼠在测试期间均减少了食物摄入量。因此，尽管在自由进食条件下明显食欲减退，但DMNL并不损害对不同质地饮食的适应能力。当给予掺有奎宁的粉末状饮食时，DMNL大鼠和对照组在9天喂养方案的第一天均明显食欲减退，但仅为短暂挑食。在一项为期16天的自选试验中，提供三种等热量饮食，每种饮食含有不同量的三种宏量营养素（HCD：高碳水化合物饮食、HFD：高脂肪饮食和HPD：高蛋白饮食），DMNL大鼠绝对摄入量（克/天）方面，HCD的摄入量相同，HFD的摄入量显著减少，在七个测量期的两个期间，HPD的摄入量也显著减少。然而，它们的摄入量占总消耗量的百分比与对照组相似。禁食48小时后，DMNL大鼠体重下降然后恢复，尽管这些大鼠在48小时再喂养期间表现出食欲减退，但其食物利用效率与对照组相似。因此，DMNL大鼠在禁食诱导体重减轻后并未丧失调节体重和食物摄入量的能力。在八次尾部夹捏（TP）试验（11天）期间，与对照组相比，DMNL大鼠食欲亢进，更喜欢HCD和HFD。在它们（实验室饲料）的饲养笼中，DMNL大鼠食欲减退，在11天内食物摄入量下降，而对照组则增加。与先前的研究结果相反，本研究的挑战揭示了DMNL大鼠在能量稳态方面的一些缺陷。
