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从携带肿瘤的仓鼠血清中免疫沉淀出的腺病毒感染细胞的蛋白激酶活性。

Protein kinase activity immunoprecipitated from adenovirus infected cells by sera from tumor-bearing hamsters.

作者信息

Branton P E, Lassam N J, Downey J F, Yee S P, Graham F L, Mak S, Bayley S T

出版信息

J Virol. 1981 Feb;37(2):601-8. doi: 10.1128/JVI.37.2.601-608.1981.

DOI:10.1128/JVI.37.2.601-608.1981
PMID:7218434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC171047/
Abstract

Earlier, we reported (N. J. Lassam, S. T. Bayley, F. L. Graham, and P. E. Branton, Nature (London) 277:241-243, 1979) detecting protein kinase activity when cytoplasmic extracts of human adenovirus type 5 (Ad5)-infected KB cells immunoprecipitated with 14b antitumor serum directed against the transforming proteins of Ad5, were incubated with [gamma-32P]ATP. Here we show that in the in vitro assay this kinase phosphorylated both the heavy chain of immunoglobulin G and polypeptide than comigrated on sodium dodecyl sulfate gels with the 58,000-dalton Ad5 antigen. It also phosphorylated added histone H3. Evidence is presented that the protein kinase activity found with extracts from Ad5-infected cells is not due to nonspecific trapping of cellular enzymes in immune complexes, but to an enzyme which is distinct from kinases detected at background levels in controls. Serine and threonine were the major phosphorylated amino acids, and essentially no phosphotyrosine was detected. Protein kinase activity detected in Ad12-infected cells immunoprecipitated by an antiserum derived from hamsters bearing Ad12-induced tumors appeared to be immunologically distinct from that immunoprecipitated from Ad5-infected cells by 14b serum.

摘要

此前，我们报道过（N. J. 拉萨姆、S. T. 贝利、F. L. 格雷厄姆和P. E. 布兰顿，《自然》（伦敦）277:241 - 243，1979），当用针对5型人腺病毒（Ad5）转化蛋白的14b抗肿瘤血清对感染Ad5的KB细胞的细胞质提取物进行免疫沉淀，然后与[γ - 32P]ATP一起孵育时，可检测到蛋白激酶活性。在此我们表明，在体外试验中，这种激酶使免疫球蛋白G的重链以及在十二烷基硫酸钠凝胶上与58,000道尔顿的Ad5抗原共迁移的多肽发生磷酸化。它还使添加的组蛋白H3发生磷酸化。有证据表明，从感染Ad5的细胞提取物中发现的蛋白激酶活性并非由于免疫复合物中非特异性捕获细胞酶，而是由于一种与对照中背景水平检测到的激酶不同的酶。丝氨酸和苏氨酸是主要的磷酸化氨基酸，基本上未检测到磷酸酪氨酸。用源自携带Ad12诱导肿瘤的仓鼠的抗血清对感染Ad12的细胞进行免疫沉淀所检测到的蛋白激酶活性，在免疫学上似乎与用14b血清从感染Ad5的细胞中免疫沉淀得到的蛋白激酶活性不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/f2ed3fd44eaf/jvirol00002-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/c4de7f62bbad/jvirol00002-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/8c9ae4bb1762/jvirol00002-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/ce1b01bbe558/jvirol00002-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/78818b4c7f5b/jvirol00002-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/f2ed3fd44eaf/jvirol00002-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/c4de7f62bbad/jvirol00002-0079-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/8c9ae4bb1762/jvirol00002-0080-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/ce1b01bbe558/jvirol00002-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/78818b4c7f5b/jvirol00002-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82ca/171047/f2ed3fd44eaf/jvirol00002-0083-a.jpg

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本文引用的文献

1
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2
Identification of the adenovirus early proteins and their genomic map positions.腺病毒早期蛋白的鉴定及其基因组图谱位置
Virology. 1980 Jan 30;100(2):419-32. doi: 10.1016/0042-6822(80)90533-4.
3
Transforming proteins of human adenovirus 5: studies with infected and transformed cells.人腺病毒5型的转化蛋白：对感染细胞和转化细胞的研究
从培养的鸡胚成纤维细胞中鉴定、纯化和表征磷酸酪氨酸特异性蛋白磷酸酶。
Mol Cell Biol. 1984 Jun;4(6):1003-12. doi: 10.1128/mcb.4.6.1003-1012.1984.
4
Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector.使用猿猴病毒40载体在猴细胞中大量生产12型腺病毒E1B基因产物。
J Virol. 1983 Jan;45(1):420-7. doi: 10.1128/JVI.45.1.420-427.1983.
5
Characterization of a polypeptide present in herpes simplex virus type 2-transformed and -infected hamster embryo cells.对单纯疱疹病毒2型转化和感染的仓鼠胚胎细胞中存在的一种多肽的特性分析。
J Virol. 1982 Mar;41(3):1095-8. doi: 10.1128/JVI.41.3.1095-1098.1982.
6
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J Muscle Res Cell Motil. 1982 Jun;3(2):191-212. doi: 10.1007/BF00711942.
7
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8
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9
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4
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7
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