Lee K Y, Nurok D, Zlatkis A
J Chromatogr. 1978 Oct 1;158:403-10. doi: 10.1016/s0021-9673(00)89983-5.
A method is described for the determination of antiarrhythmia drugs in serum by high-performance thin-layer chromatography. Baseline separations are achieved for all the drugs and clozapine, an internal standard, in two developments with solvents of different polarity. Lidocaine and diphenylhydantoin are scanned at 220 nm after the first development. Procainamide, propranolol and quinidine are scanned at 290 nm after the second development. The relative standard deviation of the determination varies between 3 and 14% depending on the nature of the drug and its concentration.
描述了一种通过高效薄层色谱法测定血清中抗心律失常药物的方法。使用不同极性的溶剂进行两次展开，可实现所有药物与内标氯氮平的基线分离。利多卡因和苯妥英在第一次展开后于220 nm处扫描。普鲁卡因胺、普萘洛尔和奎尼丁在第二次展开后于290 nm处扫描。测定的相对标准偏差根据药物的性质及其浓度在3%至14%之间变化。
