Gaiti A, Sitkievicz D, Brunetti M, Porcellati G
Neurochem Res. 1981 Jan;6(1):13-22. doi: 10.1007/BF00963901.
The incorporation of cytidine-containing precursors (CDP-Cho and CDP-Etn) into the main phospholipid classes of cellular fractions enriched in neurons and glial cells from whole rat brains of different ages was examined. The rate of synthesis of choline phosphoglycerides in neuronal homogenates significantly decreased with age up to 18 months; after this time no additional decrease was found. The decrease of CDP-Etn incorporation in neurons was found to be less significantly affected by age up to 18 months, but the enzymic activity decreased after 18 months of age. No changes were found in the corresponding glial activity at any age. Biochemical phenomena that occur in 18-month-old rat brain (aged animals) were compared with phenomena occurring in 2-month-old rat brain (adult animals). No significant variations of lipid composition were found in neurons from either 18-month-old or 2-month-old rat brain. These results, together with some kinetic parameters, suggest that ethanolamine and choline phosphotransferases are affected differently by aging.
研究了将含胞苷前体（CDP - 胆碱和CDP - 乙醇胺）掺入来自不同年龄大鼠全脑的富含神经元和神经胶质细胞的细胞组分的主要磷脂类中的情况。在神经元匀浆中，胆碱磷酸甘油酯的合成速率在18个月龄之前随年龄显著下降；在此之后未发现进一步下降。发现直到18个月龄，神经元中CDP - 乙醇胺掺入的下降受年龄影响较小，但在18个月龄后酶活性下降。在任何年龄的相应神经胶质细胞活性中均未发现变化。将18月龄大鼠脑（老龄动物）中发生的生化现象与2月龄大鼠脑（成年动物）中发生的现象进行了比较。在18月龄或2月龄大鼠脑的神经元中未发现脂质组成有显著变化。这些结果连同一些动力学参数表明，乙醇胺和胆碱磷酸转移酶受衰老的影响不同。
